Polissacarídeo sulfatado extraído da alga marinha vermelha graci- laria caudata diminui danos inflamatórios e funcionais na lesão hepática induzida por nimesulida

Abstract

Introduction: Sulphated polysaccharides comprise a group of heterogeneous and complex biopolymers from the extracellular matrix of marine algae. The sulfated polysaccharides of the red sea kelp G. caudata have been well studied due to their specific biochemical properties and characteristics, which provide some proven pharmacological effects, among which its anti-inflammatory action

is highlighted. Objective: The objective of this study was to evaluate the anti- inflammatory and antioxidant action of Gracilaria caudata sulphated polysac- charide on the effects of nimesulide-induced hepatic injury in mice. Methods:

The polysaccharide was subjected to pharmacological tests, in this study Swiss male mice weighing between 25-30g were used and divided into groups of 8-10 animals. Hepatic injury was induced using nimesulide 200mg/kg once daily for 5 consecutive days and after each induction the animals were treated with the sulfated polysaccharide of G. caudata at doses of 2.5, 5 or 10 mg / kg. After the last day of induction of hepatic lesion and treatment with the polysaccharide, sera were collected from animals and then euthanized and their livers removed for evaluation of histological scores, relative weight and biochemical dosages

such as myeloperoxidase, interleukin 1 beta, necrosis factor tumoral alpha, glu- tathione, malonyldialdehyde acid, nitrate/nitrite, as well as liver function markers

such as alanine amino transferase, aspartate amino transferase and gama glu- tamyl transferase. Results: Treatment of the animals with this polysaccharide at

a dose of 10mg/kg demonstrated a pattern of reduction of liver damage through histological visualization as well as a decrease in liver weight even. In addition,

the polysaccharide in question showed a significant reduction of all the bio- chemical tests evaluated, especially leukocyte migration markers such as

myeloperoxidase, proinflammatory cytokines (IL-1β and TNF-α), markers of ox- idative stress in vivo malonyldialdehyde, glutathione and nitrate/nitrite) and

markers of hepatic function (alanine amino transferase, aspartate amino trans- ferase and gamma glutamyl transferase). Conclusion: The results presented in

this study allow us to conclude that the anti-inflammatory action of the sulfated

polysaccharide of G. caudata has been proven, reverting the histological pa- rameters in the hemodialysis disease model induced by nimesulide. Thus, it

suggests a relation of this polysaccharide with the inhibition of the mechanism of neutrophil infiltration by mitigating the supply of pro-inflammatory cytokines and reducing oxidative stress at the sites of the lesion, in addition to restoring

liver function by restoring the levels of the markers present in the liver. This al- lows to suggest this polysaccharide as a potent candidate for the treatment of

injury resulting from the exacerbated use of nimesulide.