Avaliação De Métodos Para Conservação Laboratorial De Amostras Fúngicas Patogênicas

Abstract

Abstract: Medical mycology is an area that has gained importance, and in order to better carry out studies of fungal species, it was necessary to seek effective techniques for their preservation. There are some methods for conservation, however, none is considered the gold standard. Thus, this work aimed to evaluate three preservation methods (Cotton Balls, Castellani and Vegetal Oil) against different pathogenic fungal species. Seventeen fungal samples were used, 13 filamentous and 4 yeast. O. In the Castellani and vegetable oil methods, the fungi were previously inoculated in Sabouraud agar, grown at the appropriate time and temperature, and subsequently covered with sterile distilled water, but in the second method a small amount of sterile vegetable oil is added. In the Cotton Balls method, tubes with cotton balls were prepared, which were soaked with Sabouraud agar and subsequently received the fungi, which grew in adequate time and temperature. All methods were evaluated after periods of 6 and 24 months for viability and presence of contamination. The results showed greater effectiveness of the vegetable oil method when compared to the others, preserving

23 well both filamentous and yeast fungi, showing good growth after a period of 24 months. Castellani's method demonstrated greater efficacy in the 6-month period. The Cotton Balls method showed the worst efficiency in preserving fungi. Thus, the vegetable oil method, developed by us, proved to be the best option for the conservation of filamentous and yeast isolates in the medium term.